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Image Search Results
Journal: Bioengineered
Article Title: Long noncoding RNA matrilineal expression gene 3 inhibits hepatocellular carcinoma progression by targeting microRNA-5195-3p and regulating the expression of forkhead box O1.
doi: 10.1080/21655979.2021.2005986
Figure Lengend Snippet: Figure 4. FOXO1 is a target gene of miR-5195-3p. (a) The binding site between miR-5195-3p and FOXO1 was predicted by bioinformatics analysis. (b) Luciferase reporter assay was used to confirm the interaction between FOXO1 and miR-5195-3p. (c) RNA pull-down assay was performed to determine whether miR-5195-3p targets FOXO1. (d, e) FOXO1 expression level was detected in miR-5195-3p overexpression or knockdown group using qPCR and Western blotting. (f) qPCR was performed to evaluate the expression of FOXO1 in HCC tissues. **P < 0.01.
Article Snippet: The The membrane was blocked with 5% nonfat milk in Tris-buffered saline with Tween 20 at room temperature for 2 h and then probed with
Techniques: Binding Assay, Luciferase, Reporter Assay, Pull Down Assay, Expressing, Over Expression, Knockdown, Western Blot
Journal: Bioengineered
Article Title: Long noncoding RNA matrilineal expression gene 3 inhibits hepatocellular carcinoma progression by targeting microRNA-5195-3p and regulating the expression of forkhead box O1.
doi: 10.1080/21655979.2021.2005986
Figure Lengend Snippet: Figure 5. Co-expression of MEG3 and FOXO1 inhibits HCC progression in liver cancer cell lines. (a) qPCR was performed to detect FOXO1 expression in HepG2 and Huh7 cell lines. (b) CCK-8 was used to detect HCC cell proliferation after transfection. (c, d) Transwell assay was used to assess HCC cell migration and invasion. **P < 0.01.
Article Snippet: The The membrane was blocked with 5% nonfat milk in Tris-buffered saline with Tween 20 at room temperature for 2 h and then probed with
Techniques: Expressing, CCK-8 Assay, Transfection, Transwell Assay, Migration
Journal: Bioengineered
Article Title: Long noncoding RNA matrilineal expression gene 3 inhibits hepatocellular carcinoma progression by targeting microRNA-5195-3p and regulating the expression of forkhead box O1.
doi: 10.1080/21655979.2021.2005986
Figure Lengend Snippet: Figure 6. Xenograft tumor model demonstrates that overexpression of MEG3 inhibites the HCC progression. (a) The images of tumor in two groups. (b) The growth curve was established. (c) Tumors weight in each group was detected. (d-f) MEG3, miR-5195-3p and FOXO1 expression level was detected in the tumor tissues using qPCR. **P < 0.01, ***P < 0.001.
Article Snippet: The The membrane was blocked with 5% nonfat milk in Tris-buffered saline with Tween 20 at room temperature for 2 h and then probed with
Techniques: Over Expression, Expressing
Journal: International Journal of Molecular Sciences
Article Title: Comparison of the Chemical Components, Efficacy and Mechanisms of Action of Chrysanthemum morifolium Flower and Its Wild Relative Chrysanthemum indicum Flower against Liver-Fire Hyperactivity Syndrome of Hypertension via Integrative Analyses
doi: 10.3390/ijms232213767
Figure Lengend Snippet: Analysis of the key proteins of NF-κB and PI3K-Akt signaling pathways. ( A )—ELISA analysis of PI3K, p-PI3K, Akt, p-Akt, FOXO1, NF-κB p65, NF-κB p-p65, IκBα, respectively; ( B )—Typical bands for Western blot analysis of Akt, p-Akt, p-PI3K, NF-κB p65, and NF-κB p-p65 in C, M, CMFL, CMFH, CIFL, CIFH, P groups; ( C )—Typical bands for Western blot analysis of Akt, p-Akt, p-PI3K, NF-κB p65, and NF-κB p-p65 in C, M, CMFL, CMFM, CMFH, CIFL, CIFM, CIFH groups; ( D )—The relative expression of proteins in Western blot analysis. Values shown are means ± SD. # p < 0.05, ## p < 0.01 compared with C group; * p < 0.05, ** p < 0.01 compared with M group; ■■ p < 0.01 compared with CMFH group.
Article Snippet: Interleukin-1 beta (IL-1β, SEA563Ra), tumor necrosis factor alpha (TNF-α, SEA133Ra), interleukin-4 (IL-4, SEA077Ra), interleukin-6 (IL-6, SEA079Ra), interleukin-10 (IL-10, SEA056Ra), cyclooxygenase-2 (COX-2, SEA699Ra), angiotensin converting enzyme (ACE, SEA004Ra), and
Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Expressing
Journal: International Journal of Molecular Sciences
Article Title: Comparison of the Chemical Components, Efficacy and Mechanisms of Action of Chrysanthemum morifolium Flower and Its Wild Relative Chrysanthemum indicum Flower against Liver-Fire Hyperactivity Syndrome of Hypertension via Integrative Analyses
doi: 10.3390/ijms232213767
Figure Lengend Snippet: The differences in intervention effects on measurement indicators between CMF and CIF.
Article Snippet: Interleukin-1 beta (IL-1β, SEA563Ra), tumor necrosis factor alpha (TNF-α, SEA133Ra), interleukin-4 (IL-4, SEA077Ra), interleukin-6 (IL-6, SEA079Ra), interleukin-10 (IL-10, SEA056Ra), cyclooxygenase-2 (COX-2, SEA699Ra), angiotensin converting enzyme (ACE, SEA004Ra), and
Techniques: